Regulation and Control of Metabolism in Bacteria (page 4)
(This chapter has 5 pages)
© Kenneth Todar, PhD
Enzyme Induction
In some cases, metabolites or substrates can turn on inactive genes
so that they are transcribed. In the process of enzyme induction,
the substrate, or a compound structurally similar to the substrate,
evokes
the formation of enzyme(s) which are usually involved in the
degradation
of the substrate. Enzymes that are synthesized as a result of genes
being
turned on are called inducible enzymes and the substance that
activates
gene transcription is called the inducer. Inducible enzymes are
produced only in response to the presence of a their substrate and, in
a sense, are produced only when needed. In this way the cell does not
waste
energy synthesizing unneeded enzymes.
The best known and best studied case of enzyme induction involves
the
enzymes of lactose degradation in E. coli. Only in the presence
of lactose does the bacterium synthesize the enzymes that are necessary
to utilize lactose as a carbon and energy source for growth. Two
enzymes
are required for the initial breakdown of lactose: lactose permease,
which actively transports the sugar into the cell, and beta
galactosidase,
which splits lactose into glucose plus galactose. The genes for these
enzymes
are contained within the lactose operon (lac operon) in the
bacterial
chromosome (Figure 7).
Figure 7. The Lac operon and
its control elements
The mechanism of enzyme induction is similar to end product
repression
in that a regulatory gene, a promoter, and an operator are involved,
but
a major difference is that the lac Repressor is active only in the
absence
of the inducer molecule (lactose). In the presence of lactose, the
Repressor cannot bind to the operator region, so that the genes for
lactose
transport and cleavage are transcribed. In the absence of lactose, the
Repressor is active and will bind to the operator with the result that
the genes for lactose metabolism are not transcribed. The induction
(presence
of lactose) and the repression (absence of lactose) of the lactose
operon
is represented in Figure 8. The functions of the components and control
elements of the lac operon are shown in Table 3.
Figure 8. Enzyme Induction.
Induction (or derepression) of the lac operon.
Table 3. Functional and
regulatory
components of the lac operon
Lac I = Regulatory gene that
encodes for the lac Repressor protein that is
concerned with regulating the synthesis of the structural genes in the
operon. Lac I is adjacent to the Promoter site of the operon. An active
repressor binds to a specific nucleotide sequence in the operator
region
and thereby blocks binding of RNAp to the promoter to initiate
transcription.
The lac repressor is inactivated by lactose, and is active in the
absence
of lactose.
O = Operator specific
nucleotide
sequence on DNA to which an active Repressor binds.
P = Promoter specific
nucleotide
sequence on DNA to which RNA polymerase binds to initiate
transcription.
(The promoter site of the lac operon is further divided into two
regions,
an upstream region called the CAP site, and a downstream region
consisting
of the RNAp interaction site. The CAP site is involved in catabolite
repression
of the lac operon.). If the Repressor protein binds to the operator,
RNAp
is prevented from binding with the promoter and initiating
transcription.
Under these conditions the enzymes concerned with lactose utilization
are
not synthesized.
Lac Z, Y and A = Structural
Genes
in the lac operon. Lac Z encodes for Beta-galactosidase; Lac Y encodes
the lactose permease; Lac A encodes a transacetylase whose function is
not known.
lac = lactose, the inducer
molecule.
When lactose binds to the Repressor protein, the Repressor is
inactivated;
the operon is derepressed; the transcription of the genes for lactose
utilization
occurs.
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